Antibody engineering : methods and protocols / edited by Benny K.C. Lo

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Subjects: Immunotechnology--Laboratory manuals
Formats: Print
Material Type: Books
Language: English
Audience: Unspecified
Published: Totowa, N.J. : Humana Press, c2004
Series: Methods in molecular biology 248
Methods in molecular biology (Clifton, N.J.) v. 248
LC Classification: T, TP
Physical Description: xiv, 561 p. : ill. ; 24 cm
Table of Contents: Preface
Contributors
1. Internet Resources for the Antibody Engineer / Benny K. C. Lo, Yu Wai Chen 3
2. The Kabat Database and a Bioinformatics Example / George Johnson, Tai Te Wu 11
3. IMGT, The International ImMunoGeneTics Information System, http://imgt.cines.fr / Marie-Paule Lefranc 27
4. Antibody Variable Regions: Toward a Unified Modeling Method / Nicholas Whitelegg, Anthony R. Rees 51
5. Studying Antibody Conformations by Ultracentrifugation and Hydrodynamic Modeling / Stephen E. Harding, Emma Longman, Beatriz Carrasco, Alvaro Ortega, Jose Garcia de la Torre 93
6. PCR Cloning of Human Immunoglobulin Genes / James D. Marks, Andrew Bradbury 117
7. Antibody Humanization by CDR Grafting / Benny K. C. Lo 135
8. Selection of Human Antibodies from Phage Display Libraries / James D. Marks, Andrew Bradbury 161
9. Production of Human Single-Chain Antibodies by Ribosome Display / Mingyue He, Neil Cooley, Alison Jackson, Michael J. Taussig 177
10. Production of Human Antibodies from Transgenic Mice / C. Geoffrey Davis, Xiao-Chi Jia, Xiao Feng, Mary Haak-Frendscho 191
11. Selection of Internalizing Antibodies for Drug Delivery / James D. Marks 201
12. Engineering Multivalent Antibody Fragments for In Vivo Targeting / Anna M. Wu 209
13. Production of Recombinant Bispecific Antibodies / Roland E. Kontermann, Tina Volkel, Tina Korn 227
14. Expression and Isolation of Recombinant Antibody Fragments in E. coli / Keith A. Charlton 245
15. Expression of Recombinant Antibodies in Mammalian Cell Lines / Paul J. Yazaki, Anna M. Wu 255
16. Human Antibody Production Using Insect-Cell Expression Systems / Mary C. Guttieri, Mifang Liang 269
17. Antibody Production in Transgenic Plants / Eva Stoger, Stefan Schillberg, Richard M. Twyman, Rainer Fischer, Paul Christou 301
18. Directed Mutagenesis of Antibody Variable Domains / Kevin Brady, Benny K. C. Lo 319
19. Antibody Affinity Maturation by Chain Shuffling / James D. Marks 327
20. Antibody Affinity Maturation by Random Mutagenesis / Ikuo Fujii 345
21. Developing a Minimally Immunogenic Humanized Antibody by SDR Grafting / Syed V. S. Kashmiri, Roberto De Pascalis, Noreen R. Gonzales 361
22. Antibody Purification by Column Chromatography / Andrea Murray, C. Rosamund L. Graves, Kevin Brady, Benny K. C. Lo 379
23. Affinity Measurement Using Surface Plasmon Resonance / Robert Karlsson, Anita Larsson 389
24. Kinetic Exclusion Assays to Study High-Affinity Binding Interactions in Homogeneous Solutions / Robert C. Blake II, Diane A. Blake 417
25. Characterization of Antibody-Antigen Interactions by Fluorescence Spectroscopy / Sotiris Missailidis, Kevin Brady 431
26. Antibody Epitope Mapping Using Arrays of Synthetic Peptides / Ulrich Reineke 443
27. Mapping Antibody:Antigen Interactions by Mass Spectrometry and Bioinformatics / Alexandra Huhalov, Daniel I. R. Spencer, Kerry A. Chester 465
28. Radiometal Labeling of Antibodies and Antibody Fragments for Imaging and Therapy / Iise Novak-Hofer, Robert Waibel, Kurt Zimmermann, Roger Schibli, Jurgen Grunberg, Kerry A. Chester, Andrea Murray, Benny K. C. Lo, Alan C. Perkins, P. August Schubiger 481
29. Production and Characterization of Anti-Cocaine Catalytic Antibodies / Paloma de Prada, Donald W. Landry 495
30. Recombinant Immunotoxins in the Treatment of Cancer / Ira Pastan, Richard Beers, Tapan K. Bera 503
31. Antibodies in Proteomics / Andrew R. M. Bradbury, Nileena Velappan, Vittorio Verzillo, Milan Ovecka, Roberto Marzari, Daniele Sblattero, Leslie Chasteen, Robert Siegel, Peter Pavlik 519
32. Targeting of Antibodies Using Aptamers / Sotiris Missailidis 547
Index 557
Additional Authors: Lo, Benny K. C
Notes: LCCN: 2003020281
ISBN: 1588290921 (alk. paper)
ISBN: 1592596665 (eISBN)
Includes bibliographical references and index
OCLC Number: 53051674
ISBN/ISSN: 1588290921
1592596665